Comparison of thermodynamic stabilities between PNA (peptide nucleic acid)/DNA hybrid duplexes and DNA/DNA duplexes
نویسندگان
چکیده
منابع مشابه
Peptide nucleic acid (PNA)/DNA hybrid duplexes: intercalation by an internally linked anthraquinone.
Peptide nucleic acids (PNA) mimic DNA and RNA by forming complementary duplex structures following Watson-Crick base pairing. A set of reporter compounds that bind to DNA by intercalation are known, but these compounds do not intercalate in PNA/DNA hybrid duplexes. Analysis of the hybrid PNA duplexes requires development of reporter compounds that probe their chemical and physical properties. W...
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MOTIVATION While there are numerous programs that can predict RNA or DNA secondary structures, a program that predicts RNA/DNA hetero-dimers is still missing. The lack of easy to use tools for predicting their structure may be in part responsible for the small number of reports of biologically relevant RNA/DNA hetero-dimers. RESULTS We present here an extension to the widely used ViennaRNA Pa...
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The stability of all single-base mismatched pairs between a peptide nucleic acid 11-mer and its complementary DNA has been quantified in terms of their melting temperature and compared with the limited amount of data published to date. The strength of the interaction was determined by an automated affinity-electrophoretic approach permitting the visualization, in real time, of hybridization bet...
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Locked nucleic acids (LNA; symbols of bases, +A, +C, +G, and +T) are introduced into chemically synthesized oligonucleotides to increase duplex stability and specificity. To understand these effects, we have determined thermodynamic parameters of consecutive LNA nucleotides. We present guidelines for the design of LNA oligonucleotides and introduce free online software that predicts the stabili...
متن کاملEffect of Metal Ion Incorporation on the Properties of Peptide Nucleic Acid Duplexes
Peptide nucleic acid (PNA), a DNA synthetic analog, assembles into helical duplexes as a consequence of Watson-Crick hydrogen bonds formed between nucleobases but has an achiral pseudo-peptide backbone, in contrast to DNA which has a phosphodiester backbone. Placing ligandmodified monomers in complementary positions in PNA oligomers makes possible metal ion incorporation within the PNA duplex (...
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ژورنال
عنوان ژورنال: Nucleic Acids Symposium Series
سال: 1999
ISSN: 0261-3166,1746-8272
DOI: 10.1093/nass/42.1.93